packaging plasmid pclampho (Novus Biologicals)

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Novus Biologicals packaging plasmid pclampho
Packaging Plasmid Pclampho, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pclampho/packaging+plasmid+pclampho/pm39847828-48-23-28
Average 90 stars, based on 1 article reviews

packaging plasmid pclampho - by Bioz Stars, 2026-09

90/100 stars

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Generated:

Article Title: The melanoma‐associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair
Article Snippet: To assess lysosomal fusion with phagosomes, LysoTracker Red (Molecular Probes, Eugene, OR, USA) was applied to cells (30 min, 37°C, 200 nM). .. Amphotropic retroviruses were generated as follows: 293T17 cells in 100-mm dishes were cotransfected with pCLampho (Imgenex, San Diego, CA, USA) and pMSCV-IRESGFP, pMSCV-Gpnmb-IRES-GFP, pMXs-puro, or pMXs-GFPLC3-puro in equimolar concentrations. ..

Article Title: The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair
Article Snippet: To assess lysosomal fusion with phagosomes, LysoTracker Red (Molecular Probes, Eugene, OR, USA) was applied to cells (30 min, 37°C, 200 nM). .. Amphotropic retroviruses were generated as follows: 293T17 cells in 100-mm dishes were cotransfected with pCLampho (Imgenex, San Diego, CA, USA) and pMSCV-IRES-GFP, pMSCV-Gpnmb-IRES-GFP, pMXs-puro, or pMXs-GFPLC3-puro in equimolar concentrations. ..

Plasmid Preparation:

Article Title: Modified Hypoxia-Inducible Factor Expression in CD8+ T Cells Increases Antitumor Efficacy
Article Snippet: .. Helper vector pCL-Eco (for ecotropic infection) was a gift from Inder Verma (Addgene; plasmid #12371), and pCLAmpho (for amphotropic infection) was purchased from Novus (#NBP2-29541). .. DNA encoding a codon-optimized polycistronic peptide composed of chicken ovalbumin (OVA; P01012.2), eGFP (ADQ43426.1), and neomycin phosphotransferase (NeoR; CAD21956.1) interspersed with P2A and furin cleavage sites was synthesized by Gene Art (Thermo Fisher) and cloned under control of the SV40 promoter in the transposon vector pT2/BH, a gift from Perry Hackett (Addgene; plasmid #26556). pCMV-SB11 encoding the sleeping beauty transposase was a gift from Perry Hackett (Addgene; plasmid #26552).

Infection:

Article Title: p107 inhibits G1 to S phase progression by down-regulating expression of the F-box protein Skp2
Article Snippet: .. For infection of Rat1 populations, 293 cells were cotransfected with pBabe-puro vectors and pCLAmpho (Imgenex; 2:1 ratio) by the calcium phosphate precipitation method. ..

Cell Culture:

Article Title: Agents and methods for tissue repair and regeneration
Article Snippet: .. To generate the retrovirus, 293T17 cells were cultured in 100 mm dishes at 50% confluence, cotransfected with MSCV-Wnt7b-IRES-GFP or MSCV-IRES-GFP and pCLAmpho (Imgenex) in equal molar ratio using PEI complexes (1:4 ratio). .. After 16 hours, virus was collected in 6 ml of macrophage medium or other cell culture medium for 6 or 24 h, filtered through a 0.45 μm filter.

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